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Objective To observe heterotopic osteogenesis of autogenous marrow stromal cells on ?-tricalcium phosphate (?-TCP) scaffold, and the effects of recombinant human bone morphogenetic protein-2 (rhBMP-2) and transforming growth factor-beta (TGF-?) on osteogenesis. Methods The Newzealand rabbits autogenous marrow stromal cells were cultured by mineralized condition medium. The cell suspension was collected. All the cells with concentration of 5 ?106 ? mL-1 were divided into four groups. Group A: rhBMP-2 (15 ?g) /TGF-? (30 ng) were added; group B: TGF-? 30 ng was administered; group C: without growth factor as control; group D: continual cultured and no loading on ?-TCP. MSCs in group A, B, C were planted on ?-TCP. Alkaline phosphatase (ALP) activity was examined by histochemistry. Then MSCs loading on ?-TCP were embedded in autogenous subcutaneous. Four weeks later, the osteogenesis was observed by HE staining, type I collagen and bone morphogenetic protein synthesis were examined by immunohistochemistry stains. Results The ALP activities in groups A and B were significantly higher than those in group C and D (P
ABSTRACT
Objective: To investigate the bone healing of rigid internal fixatio n(RIF) during Le Fort I Osteotomy.Methods: We regarded rabbits as study objects and evaluated the form of bone healing by bone histomorphometry.Results:There was significant difference in the degree of the new bone formation between groups,and the degrees in group A were much higher than that in group B at any stages .Conclusion:The use of RIF in orthognathic surgery can speed the bone healing.`
ABSTRACT
Objective:To study whether the guided bone regeneration (GBR) technique can be used to achieve good bone formation in the defects of palate. Methods:Bone defect in the shape of triangle with the height of 4 cm and base line of 1.5 cm was created in the middle of palate in each of 16 adult dogs. The dogs were equally divided into experimental group(A) and control group(B). rhBMP-2/Co/ PLGA membrane was implanted in the defects in group A and the Co/ PLGA membrane in group B. The maxillary bone were observed by CT scan at 4, 8, 12 and 24 weeks after operation. The defect repair was observed by histological examination. Results:CT and histological examinations showed that new bone formation in group A was better than that in group B. 24 weeks after operation the defects in group A were completely repaired by new bone, those in group B partly repaired. Conclusion:The rhBMP-2/Co/PLGA composite membrane can be used to repair the defects in palate.